Microbiology of brewing production - bacteria of the order Enterobacterales and culture methods for their detection

Authors

  • Petra Kubizniaková Research Institute of Brewing and Malting, Plc., Lípová 511/15, Prague, Czech Republic
  • Martina Brožová Research Institute of Brewing and Malting, Plc., Lípová 511/15, Prague, Czech Republic
  • Kateřina Štulíková Research Institute of Brewing and Malting, Plc., Lípová 511/15, Prague, Czech Republic
  • Eva Vontrobová Research Institute of Brewing and Malting, Plc., Lípová 511/15, Prague, Czech Republic
  • Katarína Hanzalíková Research Institute of Brewing and Malting, Plc., Lípová 511/15, Prague, Czech Republic
  • Dagmar Matoulková Research Institute of Brewing and Malting, Plc., Lípová 511/15, Prague, Czech Republic

DOI:

https://doi.org/10.18832/kp2019.66.345

Keywords:

enterobacteria, facultative-anaerobic bacteria, Klebsiella, coliform bacteria, wort contamination, Obesumbacterium, N-nitrosamines, Rahnella, Serratia, Shimwellia

Abstract

The growth of 7 strains belonging to the order of Enterobacterales, represented by the species of Citrobacter Freundii, Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca, Obesumbacterium proteus, Rahnella aquatilis, Raoultella terrigena, Serratia marcescens and Shimwellia pseudoproteus, was monitored on selected cultivation media. Three types of agars - Endo, MacConkey and Chromocult Coliform agar together with two incubation temperatures of 28 and 37 °C were tested under aerobic conditions. The aim of the study was to detect such essential enterobacteria harmful to beer that cannot be proven at 37 °C, which is the temperature usually used in operational laboratories in breweries. Our results showed that most of the tested strains of enterobacteria were able to grow at 28 °C on all selected types of agar. The exception was just the representatives detection of which is problematic at 37 °C. Nevertheless, a little or no growth was always observed on just one of the tested media.

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Published

2020-10-15

How to Cite

Kubizniaková, P., Brožová, M., Štulíková, K., Vontrobová, E., Hanzalíková, K., & Matoulková, D. (2020). Microbiology of brewing production - bacteria of the order Enterobacterales and culture methods for their detection. KVASNY PRUMYSL, 66(5), 345-350. https://doi.org/10.18832/kp2019.66.345

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Articles