Low-Molecular Weight Proteins in Malt and Gushing
Keywords:gushing, hydrophobins, LTP proteins, SDS-PAGE
Gushing – overfoaming of beer – can be aff ected by hydrophobic polypeptides and high surface activity. The study focused on lipid transfer proteins ( LTP) and hydrophobins – products of microscopic fi lamentous fungi. For the experiment, barley samples, intermediaries of malting and malt were used. Proteins extracted from the samples were subsequently separated with the SDS-PAGE method with the TRIS-tricine buff er system. Character of the change in the content of the low-molecular weight proteins during malting was studied. The studied relationships between the low-molecular weight proteins, gushing potential and microbiological contamination of samples were not proven.