Variability and stability of essential oil composition in Ukrainian hop varieties


  • Lidia Protsenko Polissya Institute of Agriculture of NAAS of Ukraine, Shose Kyivske, 131, 10007 Zhytomyr, Ukraine
  • Liliya Janse National Academy of Agrarian Sciences of Ukraine, 9, Omelianovycha-Pavlenka Str., 01010 Kyiv, Ukraine
  • Nina Koshytska Polissya Institute of Agriculture of NAAS of Ukraine, Shose Kyivske, 131, 10007 Zhytomyr, Ukraine
  • Serhii Ryzhuk Polissya Institute of Agriculture of NAAS of Ukraine, Shose Kyivske, 131, 10007 Zhytomyr, Ukraine
  • Mykola Liashenko Polissya Institute of Agriculture of NAAS of Ukraine, Shose Kyivske, 131, 10007 Zhytomyr, Ukraine
  • Anatolii Bober National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony Str. 15, 03041 Kyiv, Ukraine
  • Sergiy Gunko National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony Str. 15, 03041 Kyiv, Ukraine



essential oil of hops, varieties of hops, variability, stability, biochemical criteria


The aim of the study was to evaluate the variation in essential oil content and composition within nine distinct Ukrainian hop varieties, with a particular focus on their genetic stability and suitability for brewing applications over 11 years of their cultivation. The hydrodistillation method (Analytica-EBC 7.10) was used to evaluate hop essential oil content and gas-liquid capillary chromatography (Analytica-EBC 7.12) to discern its qualitative composition. Variability was evaluated using statistical parameters. The essential oil content across the studied varieties spanned from 0.51 to 2.58 ml/100 g of dry hop cones. The highest content was measured in the bitter-aroma variety with a special aroma – Ruslan. The predominant essential oil components, including myrcene, caryophyllene, humulene, and farnesene, collectively constituted 70.0–84.2% of the total amount. The aroma varieties displayed myrcene content typical for European varieties (with the level up to 56.2% in the Ruslan variety). Farnesene content exhibited significant variation ranging from 0.3 to 17.6% with the highest content in Saaz-type varieties (Klon 18, Zlato Polissya, and National) and the bitter type Promin variety. Humulene content ranged from 13.2 to 32.6%. Among all studied varieties, Alta displayed the least variability for all parameters evaluated (Cv = 15.2%). This study underscores the genetic basis of essential oil composition in hops, emphasizing its potential as a biochemical criterion for identifying hop breeding varieties. The study revealed, that despite annual fluctuations, the essential oil content and composition remained within passport data declared for the variety. The findings provide valuable insight into a sustainable cultivation and optimal utilization of hops in the brewing industry.


Afonso, S., Arrobas, M., Rodrigues, Ângelo, M. (2021). Agronomic and chemical evaluation of hop cultivars grown under Mediterranean conditions. Spanish Journal of Agricultural Research, 19(3), e0904.

Analytica-EBC, 7.10 (2018). Hop Oil Content of Hops and Hop Products.

Analytica-EBC, 7.12 (2018). Hop Essential Oils by Capillary Gas Chromatography Flame Ionization Detection.

Aydin, T., Bayrak, N., Baran, E., Cakir, A. (2017). Insecticidal effects of extracts of Humulus lupulus (hops) L. cones and its principal component, xanthohumol. Bulletin of Entomological Research, 107(4), 543–549.

Bedini, S., Flamini, G., Cosci, F., Ascrizzi, R., Benelli, G., Conti, B. (2016). Cannabis sativa and Humulus lupulus essential oils as novel control tools against the invasive mosquito Aedes albopictus and fresh water snail Physella acuta. Industrial Crops and Products, 85, 318–323.

Blyznyuchenko, O. (2003). Biometrics. Poltava: Editorial and publishing department "Terra" of the Poltava State Agrarian Academy, 346 p. ISBN 966-7749-01-0

Bober, A., Liashenko, M., Protsenko, L., Slobodyanyuk, N., Matseiko, L., Yashchuk, N., Gunko, S., Mushtruk, M. (2020). Biochemical composition of the hops and quality of the finished beer. Potravinarstvo Slovak Journal of Food Sciences, 14, 307–317.

Bocquet, L., Sahpaz, S., Hilbert, J., Rambaud, C., Rivière, C. (2018a). Humulus lupulus L., a very popular beer ingredient and medicinal plant: Overview of its phytochemistry, its bioactivity, and its biotechnology. Phytochemistry Reviews, 17, 1047–1090.

Bocquet, L., Rivière, C., Dermont, C., Samaillie, J., Hilbert, J.-L., Halama, P., Siah, A., Sahpaz, S. (2018b). Antifungal activity of hop extracts and compounds against the wheat pathogen Zymoseptoria tritici. Industrial Crops and Products, 122, 290–297.

Carbone, K., Bianchi, G., Petrozziello, M., Bonello, F., Macchioni, V., Parisse, B., De Natale, F., Alilla, R., Cravero, M. (2021). Tasting the Italian Terroir through Craft Beer: Quality and Sensory Assessment of Cascade Hops Grown in Central Italy and Derived Monovarietal Beers. Foods, 10(9), 2085.

DeGrandi-Hoffman, G., Ahumada, F., Probasco, G., Schantz, L. (2012). The effects of beta acids from hops (Humulus lupulus) on mortality of Varroa destructor (Acari: Varroidae). Experimental and Applied Acarology, 58, 407–421.

Donadini, G., Porretta, S. (2017). Uncovering patterns of consumers' interest for beer: A case study with craft beers. Food Research International, 91, 183–198.

Hauser, D., Lafontaine, S., Shellhammer, Th. (2019). Extraction Efficiency of Dry-Hopping, Journal of the American Society of Brewing Chemists, 77(3), 188-198.

Hrnčič, M., Španinger, E., Košir, I., Knez, Ž., Bren, U. (2019). Hop Compounds: Extraction Techniques, Chemical Analyses, Antioxidative, Antimicrobial, and Anticarcinogenic Effects. Nutrients, 11(2), 257.

Humia, B., Santos, K., Barbosa, A., Sawata, M., Mendonça, M., Padilha, F. (2019). Beer Molecules and Its Sensory and Biological Properties: A Review. Molecules. 24(8), 1568.

Iannone, M., Ovidi, E., Vitalini, S., Masci, V., Marianelli, A., Iriti, M., Tiezzi, A., Garzoli, S. (2022). From Hops to Craft Beers: Production Process, VOCs Profile Characterization, Total Polyphenol and Flavonoid Content Determination and Antioxidant Activity Evaluation. Processes, 10(3), 517.

Isman, M.B. (2006). Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 51, 45–66.

Jirovetz, L., Bail, S., Buchbauer, G., Denkova, Z., Slavchev, A., Stoyanova, A., Schmidt, E., Geissler, M. (2006). Antimicrobial testings, gas chromatographic analysis and olfactory evaluation of an essential oil of hop cones (Humulus lupulus L.) from Bavaria and some of its main compounds. Scientia Pharmaceutica, 74, 189–201.

Karabín, M., Hudcová, T., Jelínek, L., Dostálek, P. (2016). Biologically Active Compounds from Hops and Prospects for Their Use. Comprehensive Reviews in Food Science and Food Safety, 15(3), 542–567.

Kovalev, V., Kozlik, T., Protsenko, L., Bober, A., Kormiltsev, B. (2020). Extending and maintaining the in vitro collection of (inter)national hop varieties in Ukraine. Agricultural Science and Practice, 7(3), 61–71.

Krofta, K., Klapal, I., Vojtěchová, D., Ondráček, J., Kroupa, F., Tichá, J. (2019). Hodnocení kvalitativních parametrů českých chmelů ze sklizně 2018 (eng. Evaluation of qualitative parameters of Czech hops from the harvest of 2018). Chmelařství, 92(1–2), 2–11. Available only in Czech. ISSN 0373-403X

Lamberti, L., Grillo, G., Gallina, L., Carnaroglio, D., Chemat, F., Cravotto, G. (2021). Microwave-Assisted Hydrodistillation of Hop (Humulus lupulus L.) Terpenes: A Pilot-Scale Study. Foods, 10, 2726.

Machado, Jr J., Faria, M., Ferreira, I. (2019). 10-Hops: New Perspectives for an Old Beer Ingredient. Natural Beverages, 13: The Science of Beverages, 267-301.

Nance, M., Setzer, W. (2011). Volatile components of aroma hops (Humulus lupulus L.) commonly used in beer brewing. Journal of Brewing and Distilling, 2(2), 16–22.

Nesvadba, V., Polončíková, Z., Henychová, A., Krofta, K., Patzak, J. (2012). Atlas of Czech hop varieties. Chmelařský institut s.r.o., Žatec. ISBN978-80-87357-11-8 Available from:

Nesvadba, V., Charvátová, J., Trnková, S. (2020). Breeding of flavour hops in the Czech Republic. Kvasny prumysl, 66(6), 366–371.

Nesvadba, V., Olšovská, J., Straková, L., Charvátová, J., Trnková, S. (2021). Essential oils in Czech hop varieties. Kvasny prumysl, 67(3), 447-454.

Olšovská, J., Krofta, K., Jandovská, V., Patzak J., Štěrba, K. (2016). Methods for verifying the authenticity of hops – an effective tool against falsification. Kvasny prumysl, 62(10), 294–305.

Pavela, R., Benelli, G. (2016). Essential Oils as Ecofriendly Biopesticides? Challenges and Constraints. Trends in Plant Science, 21(12),1000-1007.

Paventi, G., de Acutis, L., de Cristofaro, A., Pistillo, M., Germinara, G., Rotundo G. (2020). Biological Activity of Humulus lupulus (L.) Essential Oil and Its Main Components against Sitophilus granaries (L.) Biomolecules 10(8), 1108.

Protsenko, L., Ryzhuk, S., Liashenko, M., Shevchenko, O., Litvynchuk, S., Yanse, L., Milosta, H. (2020). Influence of alpha acids hop homologues of bitter and aromatic varieties on beer quality. Ukrainian food journal, 9(2), 425–436. doi: 10.24263/2304-974X-2020-9-2-13

Rettberg, N., Biendl, M., Garbe, L.–A. (2018). Hop Aroma and Hoppy Beer Flavor: Chemical Backgrounds and Analytical Tools – A Review. Journal of the American Society of Brewing Chemists, 76(1), 1–20.

Rutnik, K., Hrnčič, M., Košir, I. (2022a). Hop Essential Oil: Chemical Composition, Extraction, Analysis, and Applications. Food Reviews International, 38(1), 529-551.

Rutnik, K., Ocvirk, M., Košir, I. (2022b). Changes in Hop (Humulus lupulus L.) Oil Content and Composition during Long-Term Storage under Different Conditions. Foods, 11, 3089.

Salanță, L., Coldea, T., Ignat, M., Pop, C., Tofană, M., Mudura, E, Borșa, A., Pasqualone, A., Anjos, O., Zhao, H. (2020). Functionality of Special Beer Processes and Potential Health Benefits. Processes, 8(12), 1613.

Ting, P., Ryder, D. (2017). The Bitter, Twisted Truth of the Hop: 50 Years of Hop Chemistry. Journal of the American Society of Brewing Chemists, 75(3), 161–180.

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How to Cite

Protsenko, L., Janse, L. ., Koshytska, N. ., Ryzhuk, S. ., Liashenko, M. ., Bober, A., & Gunko , S. . (2023). Variability and stability of essential oil composition in Ukrainian hop varieties. KVASNY PRUMYSL, 69(6), 811-821.
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