Effect of cultivation and processing on Fusarium mycotoxins in malting barley
DOI:
https://doi.org/10.18832/kp2025.71.1065Keywords:
malting barley grain, malt, beer, mycotoxins, deoxynivalenol, zearalenone, hygienic food qualityAbstract
The study investigated the contamination of malting barley grain with mycotoxins of Fusarium during the process of growth and development of grain and its subsequent processing in the malting plant into manufactured beer. Attention was focused on their hygienic quality, zearalenone and deoxynivalenol content was determined by HPLC. None of the samples exceeded the legal limits. The experiment evaluated the influence of cultivar (Francin, Bojos), the effect of nitrogen fertilization rate and fungicide treatment. Francin appeared more resistant to ZEA occurrency, while Bojos was more resistant to DON occurency. Nitrogen fertilization rates of 80 and 110 kg/ha may have caused a higher susceptibility of the crop to Fusarium attack. The hypothesis that mycotoxin susceptibility would increase with increasing fertilization rate was not supported. The environment of the malt house may also have contributed to the increase in mycotoxin content. In the case of fungicide application, it cannot be clearly assessed whether it affected the mycotoxin content. The field experiment was adversely affected by bad weather. Differences (P < 0.05) were found when monitoring the ZEA and DON content of Bojos and Francin grain samples. The ZEA content of Bojos and Francin malt were not different (P > 0.05). Significant differences (P < 0.05) were found when monitoring the DON content of Bojos malt samples, the DON content of the Francin malt was comparable (P > 0.05). The Bojos beer samples were not different in ZEA and DON content (P > 0.05). For Francin beer, there were differences (P < 0.05).
References
Arrúa, A.A., Mendes, J.M., Arrúa, P., Ferreira F.P., Caballero, G., Cazal, C., Kohli, M.M., Peralta, I., Ulke, G., Ríos, D.F. (2019). Occurrence of deoxynivalenol and ochratoxin A in beers and wines commercialized in Paraguay. Toxins, 11(6), 308. https://doi.org/10.3390/toxins11060308
Bauer J.I., Gross, M., Gottschalk, Ch., Usleber, E. (2016). Investigations on the occurrence of mycotoxins in beer. Food Control, 63, 135–139. https://doi.org/10.1016/j.foodcont.2015.11.040
Barkei-Golan, R., Paster, N. (2008). Mycotoxins in fruits and vegetables. Academic Press, pp.395. ISBN 978-0-12-374126-4; https://doi.org/10.1016/B978-0-12-374126-4.X0001-0
Bennett, J.W., Klich, M. (2003). Mykotoxins. Clinical Microbiology Reviews, 16, 497–516. https://doi.org/10.1128/cmr.16.3.497-516.2003
Berthiller, F., Dall'Asta, C., Schuhmacher, R., Lemmens, M., Adam, G., Krska, R. (2005). Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 53(9), 3421–3425. https://doi.org/10.1021/jf047798g
Commission Regulation (EU) 2023/915 of 25 April 2023 on maximum levels for certain contaminants in food and repealing Regulation (EC) No 1881/2006
Creppy, E.E. (2002). Update of survey, regulation and toxic effects of mycotoxins. Toxikology Letters, 127(1-3), 19–28. https://doi.org/10.1016/S0378-4274(01)00479-9
Edwards, S.G. (2004). Influence of agricultural practices on fusarium infection of cereals and subsequent contamination of grain by trichothecene mycotoxins. Toxicology Letters, 153 (1), 29–35. https://doi.org/10.1016/j.toxlet.2004.04.022
Freire, L.; Sant’Ana, A.S. (2018). Modified Mycotoxins: An Updated Review on Their Formation, Detection, Occurrence, and Toxic Effects. Food Chemical Toxicology, 111, 189–205. https://doi.org/10.1016/j.fct.2017.11.021
Havránková, H., Ovesná, J. (2012). Geny biosyntézy trichothecenů u rodu Fusarium. Chemické listy, 106, 818–825. Retrieved from: http://www.chemicke-listy.cz/docs/full/2012_09_818-825.pdf
Ksieniewicz-Woźniak, E., Bryła, M., Waśkiewicz, A., Yoshinari, T., Szymczyk, K. (2019). Selected Trichothecenes in Barley Malt and Beer from Poland and an Assessment of Dietary Risks Associated with their Consumption. Toxins, 11(12), 715. https://doi.org/10.3390/toxins11120715
Lancová, K., Hajšlová, J., Poustka, J., Krplová, A., Zachariášová, M., Dostálek, P., Sachambula, L. (2008). Transfer of Fusarium mycotoxins and masked deoxynivalenol (deoxynivalenol-3-glucoside) from field barley through malt to beer. Food Additives and Contaminants, 25, 732–744. https://doi.org/10.1080/02652030701779625
Martiník, J., Boško, R., Svoboda, Z., Běláková, S., Benešová, K., Pernica, M. (2023). Determination of mycotoxins and their dietary exposure assessment in pale lager beers using immunoaffinity columns and UPLC-MS/MS. Mycotoxin Research, 39, 285–302. https://doi.org/10.1007/s12550-023-00492-4
Magan, M., Olsen, M. (2004). Mycotoxins in Food: Detection and Control. Chapter 15 – Zearalenone. Woodhead Publishing, Great Britain p. 353–356. ISBN 9781855739086
Malíř, F., Picková, D., Toman, J., Grosse, Y., Ostrý, V. (2023). Hazard characterisation for significant mycotoxins in food. Mycotoxin Research, 39, 81–93. https://doi.org/10.1007/s12550-023-00478-2
Nahle, S., El Khoury A., Atoui, A. (2021). Current status on the molecular biology of zearalenone: its biosynthesis and molecular detection of zearalenone-producing Fusarium species. European Journal of Plant Pathology, 159, 247–258. https://doi.org/10.1007/s10658-020-02177-5
Oliveira, P.M., Mauch, A., Jacob, F., Waters, D.M., Arandt, E.K. (2012). Fundamental study on the influence of Fusarium infection on quality and ultrastructure of barley malt. International Journal of Food Microbiology, 156, 32–43. https://doi.org/10.1016/j.ijfoodmicro.2012.02.019
Papadopoulou, A., Wheaton, L., Muller, R. (2000). The control of selected microorganisms during malting process. Brewing Research International, 106(3), 179–188. https://doi.org/10.1002/j.2050-0416.2000.tb00055.x
Park, A.R., Fu, M., Shin, J.Y., Son, H., Lee, Y.W. (2016). The protein kinase A pathway regulates zearalenone production by modulating alternative ZEB2 transcription. Journal of Microbiology and Biotechnology, 26(5), 967–974. https://doi.org/10.4014/jmb.1601.01032
Pascari, X., Ramos, A.J., Marín, S., Sanchís, V. (2018). Mycotoxins and beer - Impact of beer production process on mycotoxin contamination. Food Research International, 103, 121–129. https://doi.org/10.1016/j.foodres.2017.07.038
Pernica, M., Piacentini, K.C., Bosko, R., Belakova, S. (2019). The determination of deoxynivalenol and zearalenone in barley from Brazil and malted barley. MendelNet 2019 Proceedings of 26th International PhD Students Conference, Mendel University in Brno, p. 596–600. ISBN 978-80-7509-688-3
Piacentini, K.C., Běláková, S., Benešová, K., Pernica, M., Savi, G.D., Rocha, L.O., Hartman, I., Čáslavský, J., Corrêa, B. (2019). Fusarium Mycotoxins Stability during the Malting and Brewing Processes. Toxins, 11(5), 257. https://doi.org/10.3390/toxins11050257
Psota, V., Garčárová, M., Boško, R., Běláková, S., Dvořáčková, O., Dráb, Š., Krajčovič, T., Svorad, M. (2024). Ječmenářská ročenka. Research Institute of Brewing and Malting, Prague, pp. 512. ISBN 978-80-88613-41-1
Pustjens, A.M, Castenmiller, J.J.M., te Biesebeek J.D., de Rijk, T.C., van Dam R.C.J., Boon, P.E. (2022). Dietary exposure to mycotoxins of 1- and 2-year-old children from a Dutch Total Diet Study. World Mycotoxin Journal, 15, 85–97. https://doi.org/10.3920/WMJ2020.2676
Rausch, A.K., Brockmeyer R., Schwerdtle T. (2020). Development and validation of a QuEChERS-based liquid chromatography tandem mass spectrometry multi-method for the determination of 38 native and modified mycotoxins in cereals. Journal of Agricultural and Food Chemistry, 68, 4657–4669. https://doi.org/10.1021/acs.jafc.9b07491
Schwarz, P., Casper, H., Beattie, S. (1995): Fate and development of naturally occurring Fusarium mycotoxins during malting and brewing. Journal of the American Society of Brewing Chemists, 53, 121–127. https://doi.org/10.1094/ASBCJ-53-0121
Šíp, V., Chrpová, J., Leišová, L., Sýkorová, S., Kučera, L., Ovesná, J. (2007). Effects of genotype, environment and fungicide treatment on development of Fusarium head blight and accumulation of DON in winter wheat grain. Czech Journal Genetic and Plant Breeding, 43, 16–31. https://doi.org/10.17221/1905-CJGPB
Wolf-Hall, CH.E. (2007). Mold and mycotoxin problems encountered during malting and brewing. International Journal of Food Microbiology, 119(1-2), 84–89. https://doi.org/10.1016/j.ijfoodmicro.2007.07.030
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